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Multiplication of defective Ebola virus in a complementary permissive cell line.
Tong, Xian-Kun; Li, Heng; Yang, Li; Xie, Shi-Zhe; Xie, Sha; Gong, Ying; Peng, Cheng; Gao, Xiao-Xiao; Shi, Zheng-Li; Yang, Xing-Lou; Zuo, Jian-Ping.
Afiliación
  • Tong XK; State Key Laboratory of Drug Research, Immunological Disease Research Center, BSL-3 Laboratory, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China. Electronic address: xktong@simm.ac.cn.
  • Li H; State Key Laboratory of Drug Research, Immunological Disease Research Center, BSL-3 Laboratory, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
  • Yang L; State Key Laboratory of Drug Research, Immunological Disease Research Center, BSL-3 Laboratory, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.
  • Xie SZ; CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
  • Xie S; State Key Laboratory of Drug Research, Immunological Disease Research Center, BSL-3 Laboratory, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.
  • Gong Y; State Key Laboratory of Drug Research, Immunological Disease Research Center, BSL-3 Laboratory, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
  • Peng C; Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
  • Gao XX; Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
  • Shi ZL; CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
  • Yang XL; CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China; Hubei Jiangxia Lab, Wuhan, 430071, China. Electronic address: yangxinglou@mail.kiz.ac.cn.
  • Zuo JP; State Key Laboratory of Drug Research, Immunological Disease Research Center, BSL-3 Laboratory, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China. Electronic address: jpzuo@simm.ac.cn.
Antiviral Res ; 209: 105491, 2023 01.
Article en En | MEDLINE | ID: mdl-36526073
ABSTRACT
In an effort to develop safe and innovative in vitro models for Ebola virus (EBOV) research, we generated a recombinant Ebola virus where the glycoprotein (GP) gene was substituted with the Cre recombinase (Cre) gene by reverse genetics. This defective virus could multiply itself in a complementary permissive cell line, which could express GP and reporter protein upon exogenous Cre existence. The main features of this novel model for Ebola virus are intact viral life cycle, robust virus multiplication and normal virions morphology. The design of this model ensures its safety, excellent stability and maneuverability as a tool for virology research as well as for antiviral agent screening and drug discovery, and such a design could be further adapted to other viruses.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Fiebre Hemorrágica Ebola / Ebolavirus Límite: Humans Idioma: En Revista: Antiviral Res Año: 2023 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Fiebre Hemorrágica Ebola / Ebolavirus Límite: Humans Idioma: En Revista: Antiviral Res Año: 2023 Tipo del documento: Article