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Myh6 promoter-driven Cre recombinase excises floxed DNA fragments in a subset of male germline cells.
Sheldon, Caroline; Kessinger, Chase W; Sun, Yan; Kontaridis, Maria I; Ma, Qianyi; Hammoud, Saher Sue; Gao, Zibei; Zhang, Hui; Lin, Zhiqiang.
Afiliación
  • Sheldon C; Masonic Medical Research Institute, 2150 Bleecker Street, Utica, NY 13501, United States of America.
  • Kessinger CW; Masonic Medical Research Institute, 2150 Bleecker Street, Utica, NY 13501, United States of America.
  • Sun Y; Masonic Medical Research Institute, 2150 Bleecker Street, Utica, NY 13501, United States of America.
  • Kontaridis MI; Masonic Medical Research Institute, 2150 Bleecker Street, Utica, NY 13501, United States of America; Department of Medicine, Division of Cardiology, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Bos
  • Ma Q; Department of Human Genetics, University of Michigan, Ann Arbor, MI, USA.
  • Hammoud SS; Department of Human Genetics, University of Michigan, Ann Arbor, MI, USA; Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI, USA; Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI, USA; Department of Urology, University of Michigan, Ann Arbor,
  • Gao Z; School of Life Science and Technology, ShanghaiTech University, 393 Middle Huaxia Road, Pudong, Shanghai 201210, China.
  • Zhang H; School of Life Science and Technology, ShanghaiTech University, 393 Middle Huaxia Road, Pudong, Shanghai 201210, China.
  • Lin Z; Masonic Medical Research Institute, 2150 Bleecker Street, Utica, NY 13501, United States of America. Electronic address: zlin@mmri.edu.
J Mol Cell Cardiol ; 175: 62-66, 2023 02.
Article en En | MEDLINE | ID: mdl-36584478
ABSTRACT
Myh6-Cre transgenic mouse line was known to express Cre recombinase only in the heart. Nevertheless, during breeding Myh6-Cre to Rosa26fstdTom reporter (tdTom) mouse line, we observed that a significant part of their F2 tdTom/+ offspring had tdTom reporter gene universally activated. Our results show that Myh6-Cre transgenic mice have Cre recombinase activity in a subpopulation of the male germline cells, and that Myh6 gene transcripts are enriched in the interstitial Leydig cells and the undifferentiated spermatogonia stem cells. In summary, the current study confirms that the previously known "heart-specific" Myh6 promoter drives Cre expression in the testis.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Integrasas / Células Germinativas Límite: Animals Idioma: En Revista: J Mol Cell Cardiol Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Integrasas / Células Germinativas Límite: Animals Idioma: En Revista: J Mol Cell Cardiol Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos