Mass Spectrometry of Neutral Glycosphingolipids.

Suzuki, Akemi; Silsirivanit, Atit; Watanabe, Takashi; Matsuda, Junko; Inamori, Kei-Ichiro; Inokuchi, Jin-Ichi

Suzuki, Akemi; Silsirivanit, Atit; Watanabe, Takashi; Matsuda, Junko; Inamori, Kei-Ichiro; Inokuchi, Jin-Ichi.

Afiliación

Suzuki A; Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai City, Miyagi, Japan. akmszk@gaea.ocn.ne.jp.
Silsirivanit A; Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Watanabe T; Department of Pathophysiology and Metabolism, Kawasaki Medical School, Okayama, Japan.
Matsuda J; Department of Pathophysiology and Metabolism, Kawasaki Medical School, Okayama, Japan.
Inamori KI; Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai City, Miyagi, Japan.
Inokuchi JI; Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai City, Miyagi, Japan.

Methods Mol Biol ; 2613: 127-144, 2023.

Article en En | MEDLINE | ID: mdl-36587076

ABSTRACT

ABSTRACT

This chapter describes the protocols for mass spectrometry (MS) applied to the structural characterization of neutral glycosphingolipids (GSLs) and the determination of neutral GSL contents in biological materials. The structural characterization is performed by thin layer chromatography-matrix assisted laser desorption ionization/mass spectrometry (TLC-MALDI/MS) and liquid chromatography-electrospray ionization/mass spectrometry (LC-ESI/MS) with reversed phase separation. The content determination is carried out by LC-ESI/MS with multiple reaction monitoring (MRM). These protocols provide clues for the functions of neutral GSLs at the level of a single GSL molecular species.

Asunto(s)

Glicoesfingolípidos Neutros; Glicoesfingolípidos Neutros/química; Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos; Espectrometría de Masa por Ionización de Electrospray; Cromatografía Liquida; Cromatografía en Capa Delgada/métodos; Glicoesfingolípidos/química

Palabras clave

Ceramide structures; Glycan sequences; Glycosphingolipids (GSLs); Liquid chromatography-electrospray ionization mass spectrometry (LC-ESI/MS); Mass spectrometry (MS); Multiple reaction monitoring (MRM); Reversed phase liquid chromatography; Thin layer chromatography-matrix assisted laser desorption ionization/mass spectrometry (TLC-MALDI/MS)

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Glicoesfingolípidos Neutros Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article País de afiliación: Japón

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