Functional Liquid Crystal Core/Hydrogel Shell Microcapsules for Monitoring Live Cells in a 3D Microenvironment.
Anal Chem
; 95(5): 2750-2756, 2023 02 07.
Article
en En
| MEDLINE
| ID: mdl-36599406
ABSTRACT
Three-dimensional (3D) cell culture, even as a simple microspheroid model, can be used to recapitulate the native biological microenvironment of cells. Examining the biochemical characteristics of cells in multicellular hydrogel microspheroids using microsensors is usually limited to monitoring the medium around the microspheroids. Here, functional liquid crystal (LC) core/hydrogel shell microcapsules loaded with cells were prepared using droplet microfluidic technology for monitoring live cells in a 3D microenvironment. These microcapsules have a distinctive core/shell structure; cells can be cultured in the hydrogel shell of this 3D model. The functional LC core responds to the acidic microenvironment of cells, showing an axial-to-bipolar transfiguration. 3D cell culture and visual monitoring of the cell microenvironment can be simultaneously achieved in a single microcapsule. Therefore, this novel method may enable a standard approach for monitoring multiple ions or molecules in a 3D model of the cell microenvironment.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Hidrogeles
/
Cristales Líquidos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Anal Chem
Año:
2023
Tipo del documento:
Article
País de afiliación:
China