BBOX1-AS1 mediates trophoblast cells dysfunction via regulating hnRNPK/GADD45A axis.
Biol Reprod
; 108(3): 408-422, 2023 03 13.
Article
en En
| MEDLINE
| ID: mdl-36617174
ABSTRACT
Recurrent pregnancy loss (RPL) is a common pathological problem during pregnancy, and its clinical etiology is complex and unclear. Dysfunction of trophoblasts may cause a series of pregnancy complications, including preeclampsia, fetal growth restriction, and RPL. Recently, lncRNAs have been found to be closely related to the occurrence and regulation of pregnancy-related diseases, but few studies have focused on their role in RPL. In this study, we identified a novel lncRNA BBOX1-AS1 that was significantly upregulated in villous tissues and serum of RPL patients. Functionally, BBOX1-AS1 inhibited proliferation, migration, invasion, tube formation and promoted apoptosis of trophoblast cells. Mechanistically, overexpression of BBOX1-AS1 activated the p38 and JNK MAPK signaling pathways by upregulating GADD45A expression. Further studies indicated that BBOX1-AS1 could increase the stability of GADD45A mRNA by binding hnRNPK and ultimately cause abnormal trophoblast function. Collectively, our study highlights that the BBOX1-AS1/hnRNPK/GADD45A axis plays an important role in trophoblast-induced RPL and that BBOX1-AS1 may serve as a potential target for the diagnosis of RPL.
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Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Preeclampsia
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MicroARNs
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ARN Largo no Codificante
Límite:
Female
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Humans
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Pregnancy
Idioma:
En
Revista:
Biol Reprod
Año:
2023
Tipo del documento:
Article
País de afiliación:
China