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Whole Animal Feeding FLat (WAFFL): a complete and comprehensive validation of a novel, high-throughput fly experimentation system.
Jaime, Maria D L A; Salem, Ghadi H; Martinez, Daniel J; Karott, Sean; Flores, Alejandra; Palmer, Cameron D; Mahadevaraju, Sharvani; Krynitsky, Jonathan; Garmendia-Cedillos, Marcial; Anderson, Sarah; Harbison, Susan; Pohida, Thomas J; Ludington, William B; Oliver, Brian.
Afiliación
  • Jaime MDLA; Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21218, USA.
  • Salem GH; Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 50 South Drive, Bethesda, MD 20814, USA.
  • Martinez DJ; Instrument Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 12 South Dr, Bethesda, MD 20892, USA.
  • Karott S; Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21218, USA.
  • Flores A; Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 50 South Drive, Bethesda, MD 20814, USA.
  • Palmer CD; Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 50 South Drive, Bethesda, MD 20814, USA.
  • Mahadevaraju S; Department of Physiology and Biophysics, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH 44106, USA.
  • Krynitsky J; Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 50 South Drive, Bethesda, MD 20814, USA.
  • Garmendia-Cedillos M; Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 50 South Drive, Bethesda, MD 20814, USA.
  • Anderson S; Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 50 South Drive, Bethesda, MD 20814, USA.
  • Harbison S; Instrument Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 12 South Dr, Bethesda, MD 20892, USA.
  • Pohida TJ; Instrument Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 12 South Dr, Bethesda, MD 20892, USA.
  • Ludington WB; Instrument Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 12 South Dr, Bethesda, MD 20892, USA.
  • Oliver B; Laboratory of Systems Genetics, National Heart Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20814, USA.
G3 (Bethesda) ; 13(3)2023 03 09.
Article en En | MEDLINE | ID: mdl-36650008
ABSTRACT
Non-mammalian model organisms have been essential for our understanding of the mechanisms that control development, disease, and physiology, but they are underutilized in pharmacological and toxicological phenotypic screening assays due to their low throughput in comparison with cell-based screens. To increase the utility of using Drosophila melanogaster in screening, we designed the Whole Animal Feeding FLat (WAFFL), a novel, flexible, and complete system for feeding, monitoring, and assaying flies in a high-throughput format. Our 3D printed system is compatible with inexpensive and readily available, commercial 96-well plate consumables and equipment. Experimenters can change the diet at will during the experiment and video record for behavior analysis, enabling precise dosing, measurement of feeding, and analysis of behavior in a 96-well plate format.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Drosophila melanogaster / Alimentación Animal Límite: Animals Idioma: En Revista: G3 (Bethesda) Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Drosophila melanogaster / Alimentación Animal Límite: Animals Idioma: En Revista: G3 (Bethesda) Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos