MicroED in drug discovery.

Danelius, Emma; Patel, Khushboo; Gonzalez, Brenda; Gonen, Tamir

Danelius, Emma; Patel, Khushboo; Gonzalez, Brenda; Gonen, Tamir.

Afiliación

Danelius E; Department of Biological Chemistry, University of California Los Angeles, 615 Charles E.Young Drive South, Los Angeles, CA 90095, USA; Howard Hughes Medical Institute, University of California Los Angeles, Los Angeles, CA 90095, USA.
Patel K; Department of Biological Chemistry, University of California Los Angeles, 615 Charles E.Young Drive South, Los Angeles, CA 90095, USA; Howard Hughes Medical Institute, University of California Los Angeles, Los Angeles, CA 90095, USA.
Gonzalez B; Department of Biological Chemistry, University of California Los Angeles, 615 Charles E.Young Drive South, Los Angeles, CA 90095, USA; Howard Hughes Medical Institute, University of California Los Angeles, Los Angeles, CA 90095, USA.
Gonen T; Department of Biological Chemistry, University of California Los Angeles, 615 Charles E.Young Drive South, Los Angeles, CA 90095, USA; Howard Hughes Medical Institute, University of California Los Angeles, Los Angeles, CA 90095, USA; Department of Physiology, University of California Los Angeles, 61

Curr Opin Struct Biol ; 79: 102549, 2023 04.

Article en En | MEDLINE | ID: mdl-36821888

ABSTRACT

ABSTRACT

The cryo-electron microscopy (cryo-EM) method microcrystal electron diffraction (MicroED) was initially described in 2013 and has recently gained attention as an emerging technique for research in drug discovery. As compared to other methods in structural biology, MicroED provides many advantages deriving from the use of nanocrystalline material for the investigations. Here, we review the recent advancements in the field of MicroED and show important examples of small molecule, peptide and protein structures that has contributed to the current development of this method as an important tool for drug discovery.

Asunto(s)

Electrones; Proteínas; Microscopía por Crioelectrón/métodos; Modelos Moleculares; Proteínas/química; Descubrimiento de Drogas

Palabras clave

Cryo-EM; MicroED; Nanocrystals; Protein-ligand structures; Small molecule structures

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Proteínas / Electrones Idioma: En Revista: Curr Opin Struct Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos

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