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Application of Nicotinamide to Culture Medium Improves the Efficiency of Genome Editing in Hexaploid Wheat.
Wang, Wanxin; Huang, Peipei; Dai, Wenshuang; Tang, Huali; Qiu, Yuliang; Chang, Yanan; Han, Zhiyang; Li, Xi; Du, Lipu; Ye, Xingguo; Zou, Cheng; Wang, Ke.
Afiliación
  • Wang W; Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
  • Huang P; Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
  • Dai W; Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
  • Tang H; National Key Facility of Crop Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
  • Qiu Y; Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
  • Chang Y; Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
  • Han Z; Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
  • Li X; Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
  • Du L; Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
  • Ye X; Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
  • Zou C; Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
  • Wang K; National Key Facility of Crop Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Int J Mol Sci ; 24(5)2023 Feb 23.
Article en En | MEDLINE | ID: mdl-36901844
Histone acetylation is the earliest and most well-characterized of post-translation modifications. It is mediated by histone acetyltransferases (HAT) and histone deacetylases (HDAC). Histone acetylation could change the chromatin structure and status and further regulate gene transcription. In this study, nicotinamide, a histone deacetylase inhibitor (HDACi), was used to enhance the efficiency of gene editing in wheat. Transgenic immature and mature wheat embryos harboring a non-mutated GUS gene, the Cas9 and a GUS-targeting sgRNA were treated with nicotinamide in two concentrations (2.5 and 5 mM) for 2, 7, and 14 days in comparison with a no-treatment control. The nicotinamide treatment resulted in GUS mutations in up to 36% of regenerated plants, whereas no mutants were obtained from the non-treated embryos. The highest efficiency was achieved when treated with 2.5 mM nicotinamide for 14 days. To further validate the impact of nicotinamide treatment on the effectiveness of genome editing, the endogenous TaWaxy gene, which is responsible for amylose synthesis, was tested. Utilizing the aforementioned nicotinamide concentration to treat embryos containing the molecular components for editing the TaWaxy gene, the editing efficiency could be increased to 30.3% and 13.3%, respectively, for immature and mature embryos in comparison to the 0% efficiency observed in the control group. In addition, nicotinamide treatment during transformation progress could also improve the efficiency of genome editing approximately threefold in a base editing experiment. Nicotinamide, as a novel approach, may be employed to improve the editing efficacy of low-efficiency genome editing tools such as base editing and prime editing (PE) systems in wheat.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Triticum / Edición Génica Idioma: En Revista: Int J Mol Sci Año: 2023 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Triticum / Edición Génica Idioma: En Revista: Int J Mol Sci Año: 2023 Tipo del documento: Article País de afiliación: China