Genetic knockdown of genes that are obscure, conserved and essential using CRISPR interference methods in the fission yeast S. pombe.

Ishikawa, Ken; Soejima, Saeko; Saitoh, Shigeaki

Ishikawa, Ken; Soejima, Saeko; Saitoh, Shigeaki.

Afiliación

Ishikawa K; Department of Cell Biology, Institute of Life Science, Kurume University, Asahi-machi 67, Kurume, Fukuoka 830-0011, Japan.
Soejima S; Department of Cell Biology, Institute of Life Science, Kurume University, Asahi-machi 67, Kurume, Fukuoka 830-0011, Japan.
Saitoh S; Department of Cell Biology, Institute of Life Science, Kurume University, Asahi-machi 67, Kurume, Fukuoka 830-0011, Japan.

J Cell Sci ; 136(9)2023 05 01.

Article en En | MEDLINE | ID: mdl-37162093

Asunto(s)

Schizosaccharomyces; Animales; Schizosaccharomyces/genética; Proliferación Celular; Técnicas de Silenciamiento del Gen; Fenotipo; Mamíferos

Palabras clave

Biotechnology; CRISPR-Cas; CRISPRi; Essential gene; Fission yeast; Knockdown; dCas9

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Schizosaccharomyces Límite: Animals Idioma: En Revista: J Cell Sci Año: 2023 Tipo del documento: Article País de afiliación: Japón

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