Development of a Chemogenetic Approach to Manipulate Intracellular pH.
J Am Chem Soc
; 145(22): 11899-11902, 2023 06 07.
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en En
| MEDLINE
| ID: mdl-37222194
ABSTRACT
Chemogenetic Operation of iNTRacellular prOton Levels (pH-Control) is a novel substrate-based enzymatic method that enables precise spatiotemporal control of ultralocal acidification in cultured cell lines and primary neurons. The genetically encoded biosensor SypHer3s showed that pH-Control effectively acidifies cytosolic, mitochondrial, and nuclear pH exclusively in the presence of ß-chloro-d-alanine in living cells in a concentration-dependent manner. The pH-Control approach is promising for investigating the ultralocal pH imbalance associated with many diseases.
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J Am Chem Soc
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2023
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