Ratiometric optical detection of pyrophosphate based on aggregation-caused dual-signal response of gold nanoclusters.
Luminescence
; 38(8): 1458-1464, 2023 Aug.
Article
en En
| MEDLINE
| ID: mdl-37222209
ABSTRACT
Sensing of pyrophosphate ion (PPi) has received much attention due to the strong demand for clinical diagnostics. Here, based on gold nanoclusters (Au NCs), a ratiometric optical detection method for PPi is developed by simultaneously detecting the dual signals of fluorescence (FL) and second-order scattering (SOS). The PPi is detected by inhibiting the formation of aggregates of Fe3+ with Au NCs. Binding of Fe3+ to Au NCs causes aggregation of Au NCs, which leads to fluorescence quenching and scattering increasing. The presence of PPi can competitively bind Fe3+ to re-disperse the Au NCs and finally recover the fluorescence and reduce the scattering signal. The designed PPi sensor shows a high sensitivity with a linear range 5-50 µM and a detection limit of 1.2 µM. In addition, the assay has excellent selectivity for PPi, which makes its application in real biological samples extremely valuable.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Nanopartículas del Metal
/
Oro
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Luminescence
Asunto de la revista:
BIOFISICA
/
BIOQUIMICA
Año:
2023
Tipo del documento:
Article
País de afiliación:
China