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LncRNA5251 inhibits spermatogenesis via modification of cell-cell junctions.
Zhang, Cong; Lu, Dongxin; Niu, Tong; Sun, Zhongyi; Wang, Yandi; Han, Xiao; Xiong, Bohui; Shen, Wei; Sun, Qingyuan; Zhao, Yong; Zhang, Weidong; Feng, Yanni.
Afiliación
  • Zhang C; Laboratory of Animal Reproductive Physiology and Disease, College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
  • Lu D; Urology Department, Shenzhen University general hospital, Shenzhen, 518055, P. R. China.
  • Niu T; Laboratory of Animal Reproductive Physiology and Disease, College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
  • Sun Z; College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
  • Wang Y; Laboratory of Animal Reproductive Physiology and Disease, College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
  • Han X; Urology Department, Shenzhen University general hospital, Shenzhen, 518055, P. R. China.
  • Xiong B; Laboratory of Animal Reproductive Physiology and Disease, College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
  • Shen W; Laboratory of Animal Reproductive Physiology and Disease, College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
  • Sun Q; Laboratory of Animal Reproductive Physiology and Disease, College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
  • Zhao Y; College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
  • Zhang W; Fertility Preservation Lab, Reproductive Medicine Center, Guangdong Second Provincial General Hospital, Guangzhou, 510317, P. R. China.
  • Feng Y; College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
Biol Direct ; 18(1): 31, 2023 06 15.
Article en En | MEDLINE | ID: mdl-37316926
ABSTRACT

BACKGROUND:

Male factors-caused decline in total fertility has raised significant concern worldwide. LncRNAs have been identified to play various roles in biological systems, including spermatogenesis. This study aimed to explore the role of lncRNA5251 in mouse spermatogenesis.

METHODS:

The expression of lncRNA5251 was modulated in mouse testes in vivo or spermatogonial stem cells (C18-4 cells) in vitro by shRNA.

RESULTS:

The sperm motility in two generations mice after modulation of lncRNA5251 (muF0 and muF1) was decreased significantly after overexpression of lncRNA5251. GO enrichment analysis found that knockdown lncRNA5251 increased the expression of genes related to cell junctions, and genes important for spermatogenesis in mouse testes. Meanwhile, overexpressing lncRNA5251 decreased the gene and/or protein expression of important genes for spermatogenesis and immune pathways in mouse testes. In vitro, knockdown lncRNA5251 increased the expression of genes for cell junction, and the protein levels of some cell junction proteins such as CX37, OCLN, JAM1, VCAM1 and CADM2 in C18-4 cells. LncRNA5251 is involved in spermatogenesis by modulation of cell junctions.

CONCLUSION:

This will provide a theoretical basis for improving male reproductive ability via lncRNA.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Motilidad Espermática / ARN Largo no Codificante Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Biol Direct Año: 2023 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Motilidad Espermática / ARN Largo no Codificante Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Biol Direct Año: 2023 Tipo del documento: Article