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Desynchronization between Food Intake and Light Stimulations Induces Uterine Clock Quiescence in Female Mice.
Nomura, Satoshi; Hosono, Takashi; Ono, Masanori; Daikoku, Takiko; Michihiro, Mieda; Kagami, Kyosuke; Iizuka, Takashi; Chen, Yuchen; Shi, Yifan; Morishige, Jun-Ichi; Fujiwara, Tomoko; Fujiwara, Hiroshi; Ando, Hitoshi.
Afiliación
  • Nomura S; Department of Obstetrics and Gynecology, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan.
  • Hosono T; Department of Obstetrics and Gynecology, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan.
  • Ono M; Department of Obstetrics and Gynecology, Tokyo Medical University, Tokyo, Japan. Electronic address: masanori@tokyo-med.ac.jp.
  • Daikoku T; Division of Animal Disease Model, Research Center for Experimental Modeling of Human Disease, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan.
  • Michihiro M; Department of Integrative Neurophysiology, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan.
  • Kagami K; Department of Obstetrics and Gynecology, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan.
  • Iizuka T; Department of Obstetrics and Gynecology, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan.
  • Chen Y; Department of Obstetrics and Gynecology, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan.
  • Shi Y; Department of Cellular and Molecular Function Analysis, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan.
  • Morishige JI; Department of Cellular and Molecular Function Analysis, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan.
  • Fujiwara T; Department of Social Work and Life Design, Kyoto Notre Dame University, Kyoto, Japan.
  • Fujiwara H; Department of Obstetrics and Gynecology, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan. Electronic address: fuji@med.kanazawa-u.ac.jp.
  • Ando H; Department of Cellular and Molecular Function Analysis, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan. Electronic address: h-ando@med.kanazawa-u.ac.jp.
J Nutr ; 153(8): 2283-2290, 2023 08.
Article en En | MEDLINE | ID: mdl-37336322
BACKGROUND: Dysmenorrhea is associated with breakfast skipping in young women, suggesting that fasting in the early active phase disrupts uterine functions. OBJECTIVES: To investigate the possible involvement of the uterine clock system in fasting-induced uterine dysfunction, we examined core clock gene expressions in the uterus using a 28-h interval-fed mouse model. METHODS: Young female mice (8 wk of age) were divided into 3 groups: group I (ad libitum feeding), group II (time-restricted feeding, initial 4 h of the active period every day), and group III (time-restricted feeding for 8 h with a 28-h cycle). Groups II and III have the same fasting interval of 20 h. After analyzing feeding and wheel running behaviors during 2 wk of dietary restriction, mice were sacrificed at 4-h intervals, and the expression profiles of clock genes in the uterus and liver were examined by qPCR. RESULTS: The mice in group I took food mainly during the dark phase and those in group II during the initial 4 h of the dark phase, whereas those in group III delayed feeding time by 4 h per cycle. In all groups, spontaneous wheel running was observed during the dark phase. There was no difference in the quantity of feeding and the amount of running exercise among the 3 groups during the second week. The mRNA expressions of peripheral clock genes, Bmal1, Clock, Per1, Per2, Cry1, Nr1d1, and Dbp and a clock-controlled gene, Fabp1, in the uterus showed rhythmic oscillations with normal sequential expression cascade in groups I and II, whereas their expressions decreased and circadian cycles disappeared in group III. In contrast, liver core clock genes in group III showed clear circadian cycles. CONCLUSIONS: Fluctuations in the timing of the first food intake impair the uterine clock oscillator system to reduce clock gene expressions and abolish their circadian rhythms.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Ritmo Circadiano / Actividad Motora Límite: Animals Idioma: En Revista: J Nutr Año: 2023 Tipo del documento: Article País de afiliación: Japón

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Ritmo Circadiano / Actividad Motora Límite: Animals Idioma: En Revista: J Nutr Año: 2023 Tipo del documento: Article País de afiliación: Japón