New Bacteriophages Members of the Ackermannviridae Family Specific for Klebsiella pneumoniae ST258.
Phage (New Rochelle)
; 4(2): 99-107, 2023 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-37350993
ABSTRACT
Background:
Carbapenem-resistant Klebsiella pneumoniae, particularly isolates classified as sequence-type 258 (ST258), are multidrug-resistant strains that are strongly associated with poor-prognosis nosocomial infections, as current therapeutic options are limited and ineffective. In recent years, phage therapy has emerged as a promising treatment option for these scenarios. Methodology andResults:
We report the isolation and characterization of three new phages against Klebsiella pneumoniae ST258 strains recovered from Machángara river wastewater. These new members of the Ackermannviridae family showed stability over a wide temperature and pH range and burst sizes ranging from 6 to 44 plaque-forming units per bacteria. Their genomes were about 157 kilobases, with an average guanine-cytosine content of 46.4% and showed presence of several transfer RNAs, which also allowed us to predict in silico a lytic replicative cycle due to the presence of endolysins and lysozymes.Conclusion:
Three lytic phages of Ackermannviridae family were recovered against Klebsiella pneumoniae ST258 strains from sewage; however, further characterization is needed for future consideration as therapeutic alternatives.
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MEDLINE
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En
Revista:
Phage (New Rochelle)
Año:
2023
Tipo del documento:
Article
País de afiliación:
Ecuador