Efficient and versatile multiplex prime editing in hexaploid wheat.
Genome Biol
; 24(1): 156, 2023 Jun 29.
Article
en En
| MEDLINE
| ID: mdl-37386475
ABSTRACT
Prime editing is limited by low efficiency in plants. Here, we develop an upgraded engineered plant prime editor in hexaploid wheat, ePPEplus, by introducing a V223A substitution into reverse transcriptase in the ePPEmax* architecture. ePPEplus enhances the efficiency by an average 33.0-fold and 6.4-fold compared to the original PPE and ePPE, respectively. Importantly, a robust multiplex prime editing platform is established for simultaneous editing of four to ten genes in protoplasts and up to eight genes in regenerated wheat plants at frequencies up to 74.5%, thus expanding the applicability of prime editors for stacking of multiple agronomic traits.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Triticum
/
Agricultura
Idioma:
En
Revista:
Genome Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2023
Tipo del documento:
Article
País de afiliación:
China