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Methylated cycloalkanes fuel a novel genus in the Porticoccaceae family (Ca. Reddybacter gen. nov).
Arrington, Eleanor C; Tarn, Jonathan; Kittner, Hailie E; Kivenson, Veronika; Liu, Rachel M; Valentine, David L.
Afiliación
  • Arrington EC; Marine Science Institute, University of California-Santa Barbara, Santa Barbara, California, USA.
  • Tarn J; Interdepartmental Graduate Program in Marine Science, University of California-Santa Barbara, Santa Barbara, California, USA.
  • Kittner HE; Interdepartmental Graduate Program in Marine Science, University of California-Santa Barbara, Santa Barbara, California, USA.
  • Kivenson V; Innovative Genomics Institute, University of California-Berkeley, Berkeley, California, USA.
  • Liu RM; School of Oceanography, University of Washington, Seattle, Washington, USA.
  • Valentine DL; Marine Science Institute, University of California-Santa Barbara, Santa Barbara, California, USA.
Environ Microbiol ; 25(12): 2958-2971, 2023 Dec.
Article en En | MEDLINE | ID: mdl-37599091
ABSTRACT
Cycloalkanes are abundant and toxic compounds in subsurface petroleum reservoirs and their fate is important to ecosystems impacted by natural oil seeps and spills. This study focuses on the microbial metabolism of methylcyclohexane (MCH) and methylcyclopentane (MCP) in the deep Gulf of Mexico. MCH and MCP are often abundant cycloalkanes observed in petroleum and will dissolve into the water column when introduced at the seafloor via a spill or natural seep. We conducted incubations with deep Gulf of Mexico (GOM) seawater amended with MCH and MCP at four stations. Within incubations with active respiration of MCH and MCP, we found that a novel genus of bacteria belonging to the Porticoccaceae family (Candidatus Reddybacter) dominated the microbial community. Using metagenome-assembled genomes, we reconstructed the central metabolism of Candidatus Reddybacter, identifying a novel clade of the particulate hydrocarbon monooxygenase (pmo) that may play a central role in MCH and MCP metabolism. Through comparative analysis of 174 genomes, we parsed the taxonomy of the Porticoccaceae family and found evidence suggesting the acquisition of pmo and other genes related to the degradation of cyclic and branched hydrophobic compounds were likely key events in the ecology and evolution of this group of organisms.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Petróleo / Contaminación por Petróleo / Gammaproteobacteria / Cicloparafinas / Microbiota Idioma: En Revista: Environ Microbiol Asunto de la revista: MICROBIOLOGIA / SAUDE AMBIENTAL Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Petróleo / Contaminación por Petróleo / Gammaproteobacteria / Cicloparafinas / Microbiota Idioma: En Revista: Environ Microbiol Asunto de la revista: MICROBIOLOGIA / SAUDE AMBIENTAL Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos