High-throughput creation of Nicotiana tabacum gene-targeted mutants based on CRISPR/Cas9.
Plant Cell Rep
; 42(12): 2039-2042, 2023 Dec.
Article
en En
| MEDLINE
| ID: mdl-37700173
ABSTRACT
KEY MESSAGE 4382 available sgRNAs targeting 1060 tobacco genes were obtained, and 10,682 targeted mutants were created using high-throughput methods. Four optimization experiments were established to solve problems encountered during genetic transformation.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Nicotiana
/
Sistemas CRISPR-Cas
Idioma:
En
Revista:
Plant Cell Rep
Asunto de la revista:
BOTANICA
Año:
2023
Tipo del documento:
Article
País de afiliación:
China