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Integrated physiological and omics analyses reveal the mechanism of beneficial fungal Trichoderma sp. alleviating cadmium toxicity in tobacco (Nicotiana tabacum L.).
Zhang, Shuo; Zhang, Chulong; Gao, Zi-Feng; Qiu, Cheng-Wei; Shi, Shou-Heng; Chen, Zhong-Hua; Ali, Mohamed Abdelalim; Wang, Feng; Wu, Feibo.
Afiliación
  • Zhang S; Department of Agronomy, Zhejiang Key Laboratory of Crop Germplasm, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, China.
  • Zhang C; Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
  • Gao ZF; Department of Agronomy, Zhejiang Key Laboratory of Crop Germplasm, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, China.
  • Qiu CW; Department of Agronomy, Zhejiang Key Laboratory of Crop Germplasm, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, China.
  • Shi SH; Department of Agronomy, Zhejiang Key Laboratory of Crop Germplasm, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, China.
  • Chen ZH; School of Science, Hawkesbury Institute for the Environment, Western Sydney University, Penrith, NSW, Australia.
  • Ali MA; Faculty of Agriculture, Microbiology Department, Cairo University, Giza 2613, Egypt.
  • Wang F; Guizhou Academy of Tobacco Science, Guiyang 550081, China. Electronic address: yancaowangfeng@163.com.
  • Wu F; Department of Agronomy, Zhejiang Key Laboratory of Crop Germplasm, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, China. Electronic address: wufeibo@zju.edu.cn.
Ecotoxicol Environ Saf ; 267: 115631, 2023 Nov 15.
Article en En | MEDLINE | ID: mdl-37890251
Cadmium (Cd) is a highly toxic heavy metal and readily accumulates in tobacco, which imperils public health via Cd exposure from smoking. Beneficial microbes have a pivotal role in promoting plant growth, especially under environmental stresses such as heavy metal stresses. In this study, we introduced a novel fungal strain Trichoderma nigricans T32781, and investigated its capacity to alleviate Cd-induced stress in tobacco plants through comprehensive physiological and omics analyses. Our findings revealed that T32781 inoculation in soil leads to a substantial reduction in Cd-induced growth inhibition. This was evidenced by increased plant height, enhanced biomass accumulation, and improved photosynthesis, as indicated by higher values of key photosynthetic parameters, including the maximum quantum yield of photosystem Ⅱ (Fv/Fm), stomatal conductance (Gs), photosynthetic rate (Pn) and transpiration rate (Tr). Furthermore, element analysis demonstrated that T. nigricans T32781 inoculation resulted in a remarkable reduction of Cd uptake by 62.2% and a 37.8% decrease in available soil Cd compared to Cd-stressed plants without inoculation. The protective role of T32781 extended to mitigating Cd-induced oxidative stress by improving antioxidant enzyme activities of superoxide dismutase (SOD), peroxidase (POD), and ascorbate peroxidase (APX). Metabolic profiling of tobacco roots identified 43 key metabolites, with notable contributions from compounds like nicotinic acid, succinic acid, and fumaric acid in reducing Cd toxicity in T32781-inoculated plants. Additionally, rhizosphere microbiome analysis highlighted the promotion of beneficial microbes, including Gemmatimonas and Sphingomonas, by T32781 inoculation, which potentially contributed to the restoration of plant growth under Cd exposure. In summary, our study demonstrated that T. nigricans T32781 effectively alleviated Cd stress in tobacco plants by reducing Cd uptake, alleviating Cd-induced oxidative stress, influencing plant metabolite and modulating the microbial composition in the rhizosphere. These findings offer a novel perspective and a promising candidate strain for enhancing Cd tolerance and prohibiting its accumulation in plants to reduce health risks associated with exposure to Cd-contaminated plants.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Nicotiana / Trichoderma Idioma: En Revista: Ecotoxicol Environ Saf Año: 2023 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Nicotiana / Trichoderma Idioma: En Revista: Ecotoxicol Environ Saf Año: 2023 Tipo del documento: Article País de afiliación: China