Lipid peroxidation and liver damage in double and simple common bile duct ligation models in male Sprague-Dawley rats.
Life Sci
; 334: 122238, 2023 Dec 01.
Article
en En
| MEDLINE
| ID: mdl-37925139
ABSTRACT
AIMS:
Bacterial translocation, defined as the presence of living bacteria or bacterial fragments in both mesenteric lymph nodes or systemic circulation, can cause a severe inflammatory reaction in patients with cirrhosis. This study aimed to compare lipid peroxidation associated with liver damage in different experimental models of bile duct ligation proximal double ligation and transection versus proximal simple ligation versus sham. MATERIALS ANDMETHODS:
Sixty-two male rats underwent one of three bile duct surgicalinterventions:
proximal double ligation and transection (n = 22); proximal simple ligation (n = 19); or sham operation (n = 21). We performed microbiological culture of mesenteric lymph nodes; portal and cava blood, spleen and liver cultures; and histological analysis of liver parenchyma. Samples of blood and liver were obtained at laparotomy for malondialdehyde quantification. KEYFINDINGS:
Serum malondialdehyde levels were significantly higher in simple ligature animals (3.7 nmol/mg, standard deviation [SD] 2.1) compared to controls (1.6 nmol/mg SD 0.5; p = 0.001) or double ligature (0.3 nmol/mg SD 0.3; p = 0.001). Liver malondialdehyde levels were significantly higher in animals subjected to double ligation vs controls (9.0 nmol/mg SD 2.8 vs. 1.7 nmol/mg SD 1.0; p = 0.0007) and simple ligature (2.9 nmol/mg SD 2.0; p = 0.0001). Overall incidence of bacterial translocation was similar in simple and double ligatures (22.2 % and 21 % respectively), and significantly higher than in controls.SIGNIFICANCE:
the type of bile duct ligation influences the type and localization of lipid peroxidation, but does not influence the development of bacterial translocation.Palabras clave
Texto completo:
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Bases de datos:
MEDLINE
Asunto principal:
Conductos Biliares
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Hígado
Límite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Life Sci
Año:
2023
Tipo del documento:
Article