The impact of light/dark regimes on structure and physiology of Chlorella vulgaris biofilms.
Front Microbiol
; 14: 1250866, 2023.
Article
en En
| MEDLINE
| ID: mdl-37942075
ABSTRACT
Introduction:
Biofilm-based microalgae production technologies offer enormous potential for improving sustainability and productivity. However, the light pattern induced by these technologies is a key concern for optimization.Methods:
In this work, the effects of light/dark cycles on architecture, growth, and physiology of Chlorella vulgaris biofilms were assessed in a millifluidic flow-cell with different time cycles (15 s to 3 min) keeping the average light constant at 100 µmol·m-2·s-1. Results anddiscussion:
Results showed that photoinhibition can be mitigated by applying a light fraction of 1/3 and a cycle time of 15 s. By contrast, when the cycle time is extended to 90 s and 3 min, photoinhibition is high and photoefficiency dramatically decreases. To cope with light stress, cells acclimate and organize themselves differently in space. A high peak light (500 µmol·m-2·s-1) triggers a stress, reducing cell division and inducing clusters in the biofilm. This work provides guidelines for optimizing rotating microalgae production systems in biofilms and assesses the minimum rotating frequency required to maintain the net growth rate close to that of continuous light of the same average intensity, mitigating photo-inhibition. The overall gain in productivity is then provided by the total surface of the biofilm turning in the illuminated surface area.
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Bases de datos:
MEDLINE
Idioma:
En
Revista:
Front Microbiol
Año:
2023
Tipo del documento:
Article
País de afiliación:
Francia