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RAGE engagement by SARS-CoV-2 enables monocyte infection and underlies COVID-19 severity.
Angioni, Roberta; Bonfanti, Matteo; Caporale, Nicolò; Sánchez-Rodríguez, Ricardo; Munari, Fabio; Savino, Aurora; Pasqualato, Sebastiano; Buratto, Damiano; Pagani, Isabel; Bertoldi, Nicole; Zanon, Carlo; Ferrari, Paolo; Ricciardelli, Eugenia; Putaggio, Cristina; Ghezzi, Silvia; Elli, Francesco; Rotta, Luca; Scardua, Alessandro; Weber, Janine; Cecatiello, Valentina; Iorio, Francesco; Zonta, Francesco; Cattelan, Anna Maria; Vicenzi, Elisa; Vannini, Alessandro; Molon, Barbara; Villa, Carlo Emanuele; Viola, Antonella; Testa, Giuseppe.
Afiliación
  • Angioni R; Department of Biomedical Sciences, University of Padova, 35131 Padova, Italy; Fondazione Istituto di Ricerca Pediatrica - Città Della Speranza, 35127 Padova, Italy.
  • Bonfanti M; Human Technopole, Viale Rita Levi-Montalcini 1, 20157 Milan, Italy.
  • Caporale N; Human Technopole, Viale Rita Levi-Montalcini 1, 20157 Milan, Italy; Department of Oncology and Hemato-Oncology, University of Milan, Via Santa Sofia 9, 20122 Milan, Italy; Department of Experimental Oncology, European Institute of Oncology IRCCS, Via Adamello 16, 20139 Milan, Italy.
  • Sánchez-Rodríguez R; Department of Biomedical Sciences, University of Padova, 35131 Padova, Italy; Fondazione Istituto di Ricerca Pediatrica - Città Della Speranza, 35127 Padova, Italy.
  • Munari F; Department of Biomedical Sciences, University of Padova, 35131 Padova, Italy; Fondazione Istituto di Ricerca Pediatrica - Città Della Speranza, 35127 Padova, Italy.
  • Savino A; Human Technopole, Viale Rita Levi-Montalcini 1, 20157 Milan, Italy.
  • Pasqualato S; Human Technopole, Viale Rita Levi-Montalcini 1, 20157 Milan, Italy.
  • Buratto D; Institute of Quantitative Biology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China; Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China.
  • Pagani I; Viral Pathogenesis and Biosafety Unit, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy.
  • Bertoldi N; Department of Biomedical Sciences, University of Padova, 35131 Padova, Italy; Fondazione Istituto di Ricerca Pediatrica - Città Della Speranza, 35127 Padova, Italy.
  • Zanon C; Fondazione Istituto di Ricerca Pediatrica - Città Della Speranza, 35127 Padova, Italy.
  • Ferrari P; Human Technopole, Viale Rita Levi-Montalcini 1, 20157 Milan, Italy.
  • Ricciardelli E; Human Technopole, Viale Rita Levi-Montalcini 1, 20157 Milan, Italy.
  • Putaggio C; Infectious Disease Unit, Padova University Hospital, 35128 Padova, Italy.
  • Ghezzi S; Viral Pathogenesis and Biosafety Unit, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy.
  • Elli F; Human Technopole, Viale Rita Levi-Montalcini 1, 20157 Milan, Italy; Department of Oncology and Hemato-Oncology, University of Milan, Via Santa Sofia 9, 20122 Milan, Italy.
  • Rotta L; Department of Experimental Oncology, European Institute of Oncology IRCCS, Via Adamello 16, 20139 Milan, Italy.
  • Scardua A; Human Technopole, Viale Rita Levi-Montalcini 1, 20157 Milan, Italy.
  • Weber J; Human Technopole, Viale Rita Levi-Montalcini 1, 20157 Milan, Italy.
  • Cecatiello V; Human Technopole, Viale Rita Levi-Montalcini 1, 20157 Milan, Italy.
  • Iorio F; Human Technopole, Viale Rita Levi-Montalcini 1, 20157 Milan, Italy.
  • Zonta F; Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; Department of Biological Sciences, Xi'an Jiaotong-Liverpool University, Suzhou 215123, China.
  • Cattelan AM; Infectious Disease Unit, Padova University Hospital, 35128 Padova, Italy.
  • Vicenzi E; Viral Pathogenesis and Biosafety Unit, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy.
  • Vannini A; Human Technopole, Viale Rita Levi-Montalcini 1, 20157 Milan, Italy.
  • Molon B; Department of Biomedical Sciences, University of Padova, 35131 Padova, Italy; Fondazione Istituto di Ricerca Pediatrica - Città Della Speranza, 35127 Padova, Italy.
  • Villa CE; Human Technopole, Viale Rita Levi-Montalcini 1, 20157 Milan, Italy; Department of Experimental Oncology, European Institute of Oncology IRCCS, Via Adamello 16, 20139 Milan, Italy.
  • Viola A; Department of Biomedical Sciences, University of Padova, 35131 Padova, Italy; Fondazione Istituto di Ricerca Pediatrica - Città Della Speranza, 35127 Padova, Italy. Electronic address: antonella.viola@unipd.it.
  • Testa G; Human Technopole, Viale Rita Levi-Montalcini 1, 20157 Milan, Italy; Department of Oncology and Hemato-Oncology, University of Milan, Via Santa Sofia 9, 20122 Milan, Italy; Department of Experimental Oncology, European Institute of Oncology IRCCS, Via Adamello 16, 20139 Milan, Italy. Electronic addre
Cell Rep Med ; 4(11): 101266, 2023 11 21.
Article en En | MEDLINE | ID: mdl-37944530
The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has fueled the COVID-19 pandemic with its enduring medical and socioeconomic challenges because of subsequent waves and long-term consequences of great concern. Here, we chart the molecular basis of COVID-19 pathogenesis by analyzing patients' immune responses at single-cell resolution across disease course and severity. This approach confirms cell subpopulation-specific dysregulation in COVID-19 across disease course and severity and identifies a severity-associated activation of the receptor for advanced glycation endproducts (RAGE) pathway in monocytes. In vitro THP1-based experiments indicate that monocytes bind the SARS-CoV-2 S1-receptor binding domain (RBD) via RAGE, pointing to RAGE-Spike interaction enabling monocyte infection. Thus, our results demonstrate that RAGE is a functional receptor of SARS-CoV-2 contributing to COVID-19 severity.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: COVID-19 Límite: Humans Idioma: En Revista: Cell Rep Med Año: 2023 Tipo del documento: Article País de afiliación: Italia

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: COVID-19 Límite: Humans Idioma: En Revista: Cell Rep Med Año: 2023 Tipo del documento: Article País de afiliación: Italia