Chemical constitutes from Tuber indicum with immunosuppressive activity uncovered by transcriptome analysis.
Fitoterapia
; 173: 105773, 2024 Mar.
Article
en En
| MEDLINE
| ID: mdl-38097020
ABSTRACT
Three previously undescribed compounds including a polyketide (1) and two lactams (2 and 3) were obtained from Tuber indicum. The structures of new findings were elucidated by HRESIMS, NMR as well as NMR and ECD calculations. Transcriptome analysis through RNA-seq revealed that compound 2 exhibits immunosuppressive activity. Lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were employed as a model to explore the effect of these compounds in immunosuppressive activity. The results showed that 2 could reduce the generation of inflammatory mediators including nitric oxide (NO), reactive oxygen species (ROS), interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS). Western blotting analysis demonstrated that 2 could suppressed the PI3K pathway by decreasing the levels of p-PI3K and p-Akt, while increasing the levels of p-PTEN. The anti-inflammatory activity of 2 was further confirmed using a zebrafish in vivo model.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Ascomicetos
/
FN-kappa B
/
Fosfatidilinositol 3-Quinasas
Límite:
Animals
Idioma:
En
Revista:
Fitoterapia
Año:
2024
Tipo del documento:
Article
País de afiliación:
China