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Reveal the kernel dehydration mechanisms in maize based on proteomic and metabolomic analysis.
Zhang, Hao; Gou, Xiaonan; Ma, Liangchuan; Zhang, Xiaojun; Qu, Jianzhou; Wang, Xiaoyue; Huang, Wenjie; Yan, Shijuan; Zhang, Xinghua; Xue, Jiquan; Xu, Shutu.
Afiliación
  • Zhang H; Key Laboratory of Biology and Genetic Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture and Rural Affairs, College of Agronomy, Northwest A&F University, Shaanxi, 712100, Yangling, China.
  • Gou X; Maize Engineering Technology Research Centre, Shaanxi, 712100, Yangling, China.
  • Ma L; Key Laboratory of Biology and Genetic Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture and Rural Affairs, College of Agronomy, Northwest A&F University, Shaanxi, 712100, Yangling, China.
  • Zhang X; Maize Engineering Technology Research Centre, Shaanxi, 712100, Yangling, China.
  • Qu J; Key Laboratory of Biology and Genetic Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture and Rural Affairs, College of Agronomy, Northwest A&F University, Shaanxi, 712100, Yangling, China.
  • Wang X; Maize Engineering Technology Research Centre, Shaanxi, 712100, Yangling, China.
  • Huang W; Key Laboratory of Biology and Genetic Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture and Rural Affairs, College of Agronomy, Northwest A&F University, Shaanxi, 712100, Yangling, China.
  • Yan S; Maize Engineering Technology Research Centre, Shaanxi, 712100, Yangling, China.
  • Zhang X; Key Laboratory of Biology and Genetic Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture and Rural Affairs, College of Agronomy, Northwest A&F University, Shaanxi, 712100, Yangling, China.
  • Xue J; Maize Engineering Technology Research Centre, Shaanxi, 712100, Yangling, China.
  • Xu S; Key Laboratory of Biology and Genetic Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture and Rural Affairs, College of Agronomy, Northwest A&F University, Shaanxi, 712100, Yangling, China.
BMC Plant Biol ; 24(1): 15, 2024 Jan 02.
Article en En | MEDLINE | ID: mdl-38163910
ABSTRACT

BACKGROUND:

Kernel dehydration is an important factor for the mechanized harvest in maize. Kernel moisture content (KMC) and kernel dehydration rate (KDR) are important indicators for kernel dehydration. Although quantitative trait loci and genes related to KMC have been identified, where most of them only focus on the KMC at harvest, these are still far from sufficient to explain all genetic variations, and the relevant regulatory mechanisms are still unclear. In this study, we tried to reveal the key proteins and metabolites related to kernel dehydration in proteome and metabolome levels. Moreover, we preliminarily explored the relevant metabolic pathways that affect kernel dehydration combined proteome and metabolome. These results could accelerate the development of further mechanized maize technologies.

RESULTS:

In this study, three maize inbred lines (KB182, KB207, and KB020) with different KMC and KDR were subjected to proteomic analysis 35, 42, and 49 days after pollination (DAP). In total, 8,358 proteins were quantified, and 2,779 of them were differentially expressed proteins in different inbred lines or at different stages. By comparative analysis, K-means cluster, and weighted gene co-expression network analysis based on the proteome data, some important proteins were identified, which are involved in carbohydrate metabolism, stress and defense response, lipid metabolism, and seed development. Through metabolomics analysis of KB182 and KB020 kernels at 42 DAP, 18 significantly different metabolites, including glucose, fructose, proline, and glycerol, were identified.

CONCLUSIONS:

In sum, we inferred that kernel dehydration could be regulated through carbohydrate metabolism, antioxidant systems, and late embryogenesis abundant protein and heat shock protein expression, all of which were considered as important regulatory factors during kernel dehydration process. These results shed light on kernel dehydration and provide new insights into developing cultivars with low moisture content.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Zea mays / Deshidratación Tipo de estudio: Prognostic_studies Idioma: En Revista: BMC Plant Biol Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Zea mays / Deshidratación Tipo de estudio: Prognostic_studies Idioma: En Revista: BMC Plant Biol Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: China