The interaction of Synapsin 2a and Synaptogyrin-3 regulates fear extinction in mice.
J Clin Invest
; 134(4)2024 Jan 04.
Article
en En
| MEDLINE
| ID: mdl-38175724
ABSTRACT
The mechanisms behind a lack of efficient fear extinction in some individuals are unclear. Here, by employing a principal components analysis-based approach, we differentiated the mice into extinction-resistant and susceptible groups. We determined that elevated synapsin 2a (Syn2a) in the infralimbic cortex (IL) to basolateral amygdala (BLA) circuit disrupted presynaptic orchestration, leading to an excitatory/inhibitory imbalance in the BLA region and causing extinction resistance. Overexpression or silencing of Syn2a levels in IL neurons replicated or alleviated behavioral, electrophysiological, and biochemical phenotypes in resistant mice. We further identified that the proline-rich domain H in the C-terminus of Syn2a was indispensable for the interaction with synaptogyrin-3 (Syngr3) and demonstrated that disrupting this interaction restored extinction impairments. Molecular docking revealed that ritonavir, an FDA-approved HIV drug, could disrupt Syn2a-Syngr3 binding and rescue fear extinction behavior in Syn2a-elevated mice. In summary, the aberrant elevation of Syn2a expression and its interaction with Syngr3 at the presynaptic site were crucial in fear extinction resistance, suggesting a potential therapeutic avenue for related disorders.
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Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Corteza Prefrontal
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Miedo
Límite:
Animals
Idioma:
En
Revista:
J Clin Invest
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J. clin. invest
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Journal of clinical investigation
Año:
2024
Tipo del documento:
Article
País de afiliación:
China