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The type VI secretion system of Stenotrophomonas rhizophila CFBP13503 limits the transmission of Xanthomonas campestris pv. campestris 8004 from radish seeds to seedlings.
Garin, Tiffany; Brin, Chrystelle; Préveaux, Anne; Brault, Agathe; Briand, Martial; Simonin, Marie; Barret, Matthieu; Journet, Laure; Sarniguet, Alain.
Afiliación
  • Garin T; Univ Angers, Institut Agro, INRAE, IRHS, SFR QUASAV, Angers, France.
  • Brin C; Univ Angers, Institut Agro, INRAE, IRHS, SFR QUASAV, Angers, France.
  • Préveaux A; Univ Angers, Institut Agro, INRAE, IRHS, SFR QUASAV, Angers, France.
  • Brault A; Univ Angers, Institut Agro, INRAE, IRHS, SFR QUASAV, Angers, France.
  • Briand M; Univ Angers, Institut Agro, INRAE, IRHS, SFR QUASAV, Angers, France.
  • Simonin M; Univ Angers, Institut Agro, INRAE, IRHS, SFR QUASAV, Angers, France.
  • Barret M; Univ Angers, Institut Agro, INRAE, IRHS, SFR QUASAV, Angers, France.
  • Journet L; Laboratoire d'Ingénierie des Systèmes Macromoléculaires, Institut de Microbiologie, Bioénergies et Biotechnologie, Institut de Microbiologie de la Méditerranée, Aix-Marseille Université-CNRS, UMR 7255, Marseille, France.
  • Sarniguet A; Univ Angers, Institut Agro, INRAE, IRHS, SFR QUASAV, Angers, France.
Mol Plant Pathol ; 25(1): e13412, 2024 Jan.
Article en En | MEDLINE | ID: mdl-38279854
ABSTRACT
Stenotrophomonas rhizophila CFBP13503 is a seedborne commensal bacterial strain, which is efficiently transmitted to seedlings and can outcompete the phytopathogenic bacterium Xanthomonas campestris pv. campestris (Xcc8004). The type VI secretion system (T6SS), an interference contact-dependent mechanism, is a critical component of interbacterial competition. The involvement of the T6SS of S. rhizophila CFBP13503 in the inhibition of Xcc8004 growth and seed-to-seedling transmission was assessed. The T6SS cluster of S. rhizophila CFBP13503 and nine putative effectors were identified. Deletion of two T6SS structural genes, hcp and tssB, abolished the competitive advantage of S. rhizophila against Xcc8004 in vitro. The population sizes of these two bacterial species were monitored in seedlings after inoculation of radish seeds with mixtures of Xcc8004 and either S. rhizophila wild-type (wt) strain or isogenic hcp mutant. A significant decrease in the population size of Xcc8004 was observed during confrontation with the S. rhizophila wt in comparison with T6SS-deletion mutants in germinated seeds and seedlings. We found that the T6SS distribution among 835 genomes of the Stenotrophomonas genus is scarce. In contrast, in all available S. rhizophila genomes, T6SS clusters are widespread and mainly belong to the T6SS group i4. In conclusion, the T6SS of S. rhizophila CFBP13503 is involved in the antibiosis against Xcc8004 and reduces seedling transmission of Xcc8004 in radish. The distribution of this T6SS cluster in the S. rhizophila complex could make it possible to exploit these strains as biocontrol agents against X. campestris pv. campestris.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Xanthomonas campestris / Raphanus / Sistemas de Secreción Tipo VI Idioma: En Revista: Mol Plant Pathol Año: 2024 Tipo del documento: Article País de afiliación: Francia

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Xanthomonas campestris / Raphanus / Sistemas de Secreción Tipo VI Idioma: En Revista: Mol Plant Pathol Año: 2024 Tipo del documento: Article País de afiliación: Francia