Cargo-specific effects of hypoxia on clathrin-mediated trafficking.
Pflugers Arch
; 476(9): 1399-1410, 2024 Sep.
Article
en En
| MEDLINE
| ID: mdl-38294517
ABSTRACT
Clathrin-associated trafficking is a major mechanism for intracellular communication, as well as for cells to communicate with the extracellular environment. A decreased oxygen availability termed hypoxia has been described to influence this mechanism in the past. Mostly biochemical studies were applied in these analyses, which miss spatiotemporal information. We have applied live cell microscopy and a newly developed analysis script in combination with a GFP-tagged clathrin-expressing cell line to obtain insight into the dynamics of the effect of hypoxia. Number, mobility and directionality of clathrin-coated vesicles were analysed in non-stimulated cells as well as after stimulation with epidermal growth factor (EGF) or transferrin in normoxic and hypoxic conditions. These data reveal cargo-specific effects, which would not be observable with biochemical methods or with fixed cells and add to the understanding of cell physiology in hypoxia. The stimulus-dependent consequences were also reflected in the final cellular output, i.e. decreased EGF signaling and in contrast increased iron uptake in hypoxia.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Transferrina
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Hipoxia de la Célula
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Clatrina
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Vesículas Cubiertas por Clatrina
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Factor de Crecimiento Epidérmico
Límite:
Animals
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Humans
Idioma:
En
Revista:
Pflugers Arch
Año:
2024
Tipo del documento:
Article
País de afiliación:
Alemania