Contribution of sulfur-containing precursors to release of hydrogen sulfide in sludge composting.

ABSTRACT

Hydrogen sulfide (H2S) production during composting can impact the environment and human health. Especially during the thermophilic phase, H2S is discharged in large quantities. However, in sludge composting, the contributions of different sulfur-containing precursors to H2S fluxes, key functional microorganisms, and key environmental parameters for reducing H2S flux remain unclear. Analysis of cysteine (Cys), methionine (Met), and sulfate (SO42-) concentrations, multiple stepwise regression analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation analysis of metagenomes showed that Cys was the main contributor to the production of H2S and that Met was among the main sources during the first three days of composting, while the SO42- contribution to H2S was negligible. Fifteen functional genera involved in the conversion of precursors to H2S were identified by co-occurrence network analysis. Only Bacillus showed high temperature resistance (>50 °C) and the ability to reduce H2S. Redundancy analysis showed that total carbon (64.0 %) and pH (23.3 %) had significant effects on functional bacteria. H2S had a quadratic relationship with sulfur-containing precursors. All microbial network sulfur-containing precursors metabolism modules showed a highly significant relationship with Cys.