In vitro evaluation of the antitumor activity of axitinib in canine mammary gland tumor cell lines.
J Vet Sci
; 25(1): e1, 2024 Jan.
Article
en En
| MEDLINE
| ID: mdl-38311316
ABSTRACT
BACKGROUND:
Axitinib, a potent and selective inhibitor of vascular endothelial growth factor (VEGF) receptor (VEGFR) tyrosine kinase 1,2 and 3, is used in chemotherapy because it inhibits tumor angiogenesis by blocking the VEGF/VEGFR pathway. In veterinary medicine, attempts have been made to apply tyrosine kinase inhibitors with anti-angiogenic effects to tumor patients, but there are no studies on axitinib in canine mammary gland tumors (MGTs).OBJECTIVES:
This study aimed to confirm the antitumor activity of axitinib in canine mammary gland cell lines.METHODS:
We treated canine MGT cell lines (CIPp and CIPm) with axitinib and conducted CCK, wound healing, apoptosis, and cell cycle assays. Additionally, we evaluated the expression levels of angiogenesis-associated factors, including VEGFs, PDGF-A, FGF-2, and TGF-ß1, using quantitative real-time polymerase chain reaction. Furthermore, we collected canine peripheral blood mononuclear cells (PBMCs), activated them with concanavalin A (ConA) and lipopolysaccharide (LPS), and then treated them with axitinib to investigate changes in viability.RESULTS:
When axitinib was administered to CIPp and CIPm, cell viability significantly decreased at 24, 48, and 72 h (p < 0.001), and migration was markedly reduced (6 h, p < 0.05; 12 h, p < 0.005). The apoptosis rate significantly increased (p < 0.01), and the G2/M phase ratio showed a significant increase (p < 0.001). Additionally, there was no significant change in the viability of canine PBMCs treated with LPS and ConA.CONCLUSION:
In this study, we confirmed the antitumor activity of axitinib against canine MGT cell lines. Accordingly, we suggest that axitinib can be applied as a new treatment for patients with canine MGTs.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Glándulas Mamarias Humanas
/
Factor A de Crecimiento Endotelial Vascular
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Vet Sci
/
J. vet. sci
/
Journal of veterinary science
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2024
Tipo del documento:
Article