Functional validation of novel levamisole resistance marker S168T in Haemonchus contortus.
Int J Parasitol Drugs Drug Resist
; 24: 100524, 2024 Apr.
Article
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| MEDLINE
| ID: mdl-38346379
ABSTRACT
Recently, a S168T variant in the acetylcholine receptor subunit ACR-8 was associated with levamisole resistance in the parasitic helminth Haemonchus contortus. Here, we used the Xenopus laevis oocyte expression system and two-electrode voltage-clamp electrophysiology to measure the functional impact of this S168T variant on the H. contortus levamisole-sensitive acetylcholine receptor, L-AChR-1.1. Expression of the ACR-8 S168T variant significantly reduced the current amplitude elicited by levamisole compared to acetylcholine, with levamisole changing from a full to partial agonist on the recombinant L-AChR. Functional validation of the S168T mutation on modulating levamisole activity at the receptor level highlights its critical importance as both a mechanism and a marker of levamisole resistance.
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Bases de datos:
MEDLINE
Asunto principal:
Parásitos
/
Haemonchus
/
Antihelmínticos
Límite:
Animals
Idioma:
En
Revista:
Int J Parasitol Drugs Drug Resist
Año:
2024
Tipo del documento:
Article