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The serine-threonine protein kinase Snf1 orchestrates the expression of plant cell wall-degrading enzymes and is required for full virulence of the maize pathogen Colletotrichum graminicola.
de Oliveira Silva, Alan; Fernando Devasahayam, Bennet Rohan; Aliyeva-Schnorr, Lala; Glienke, Chirlei; Deising, Holger B.
Afiliación
  • de Oliveira Silva A; Chair of Phytopathology and Plant Protection, Institute for Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Germany; Department of Genetics, Federal University of Paraná, Curitiba, PR, Brazil.
  • Fernando Devasahayam BR; Chair of Phytopathology and Plant Protection, Institute for Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Germany; German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Germany.
  • Aliyeva-Schnorr L; Chair of Phytopathology and Plant Protection, Institute for Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Germany.
  • Glienke C; Department of Genetics, Federal University of Paraná, Curitiba, PR, Brazil.
  • Deising HB; Chair of Phytopathology and Plant Protection, Institute for Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Germany; German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Germany. Electronic address: holger.deising@landw.uni-halle.de.
Fungal Genet Biol ; 171: 103876, 2024 03.
Article en En | MEDLINE | ID: mdl-38367799
ABSTRACT
Colletotrichum graminicola, the causal agent of maize leaf anthracnose and stalk rot, differentiates a pressurized infection cell called an appressorium in order to invade the epidermal cell, and subsequently forms biotrophic and necrotrophic hyphae to colonize the host tissue. While the role of force in appressorial penetration is established (Bechinger et al., 1999), the involvement of cell wall-degrading enzymes (CWDEs) in this process and in tissue colonization is poorly understood, due to the enormous number and functional redundancy of these enzymes. The serine/threonine protein kinase gene SNF1 identified in Sucrose Non-Fermenting yeast mutants mediates de-repression of catabolite-repressed genes, including many genes encoding CWDEs. In this study, we identified and functionally characterized the SNF1 homolog of C. graminicola. Δsnf1 mutants showed reduced vegetative growth and asexual sporulation rates on media containing polymeric carbon sources. Microscopy revealed reduced efficacies in appressorial penetration of cuticle and epidermal cell wall, and formation of unusual medusa-like biotrophic hyphae by Δsnf1 mutants. Severe and moderate virulence reductions were observed on intact and wounded leaves, respectively. Employing RNA-sequencing we show for the first time that more than 2,500 genes are directly or indirectly controlled by Snf1 in necrotrophic hyphae of a plant pathogenic fungus, many of which encode xylan- and cellulose-degrading enzymes. The data presented show that Snf1 is a global regulator of gene expression and is required for full virulence.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Zea mays / Colletotrichum Idioma: En Revista: Fungal Genet Biol Asunto de la revista: GENETICA / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Brasil

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Zea mays / Colletotrichum Idioma: En Revista: Fungal Genet Biol Asunto de la revista: GENETICA / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Brasil