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Isolation of total DNA from Placenta Samples, both Fresh and following Formalin and Paraffin Treatment.
Villavicencio-Carrisoza, Oscar; Mora-Vargas, Carlos Daniel; Flores-Villanueva, Janet; Martínez-Salazar, María Guadalupe; Helguera-Repetto, Addy Cecilia.
Afiliación
  • Villavicencio-Carrisoza O; Department of Immunobiochemistry, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Mexico City, Mexico.
  • Mora-Vargas CD; Department of Immunobiochemistry, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Mexico City, Mexico.
  • Flores-Villanueva J; Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico.
  • Martínez-Salazar MG; Department of Immunobiochemistry, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Mexico City, Mexico.
  • Helguera-Repetto AC; Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico.
Methods Mol Biol ; 2781: 171-178, 2024.
Article en En | MEDLINE | ID: mdl-38502453
ABSTRACT
The isolation of DNA from placental tissue suspected of infection is an important tool for identifying microorganisms such as bacteria, fungi, and viruses associated with complications during and after pregnancy. While experts primarily process placental tissue, the preservation methods employed pose challenges to extracting complete DNA. Therefore, selecting the appropriate protocol is paramount to achieving greater efficiency in obtaining genetic material.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Placenta / Formaldehído Límite: Female / Humans / Pregnancy Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: México

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Placenta / Formaldehído Límite: Female / Humans / Pregnancy Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: México