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A Multiplex "Disposable Photonics" Biosensor Platform and Its Application to Antibody Profiling in Upper Respiratory Disease.
Bryan, Michael R; Butt, Jordan N; Ding, Zhong; Tokranova, Natalya; Cady, Nathaniel; Piorek, Brian; Meinhart, Carl; Tice, Joshua; Miller, Benjamin L.
Afiliación
  • Bryan MR; Department of Dermatology, University of Rochester, Rochester, New York 14627, United States.
  • Butt JN; Department of Biochemistry and Biophysics, University of Rochester, Rochester, New York 14627, United States.
  • Ding Z; Department of Chemistry, University of Rochester, Rochester, New York 14627, United States.
  • Tokranova N; ZDing Tech, LLC, Pittsford, New York 14534, United States.
  • Cady N; Department of Nanoscale Science & Engineering, University at Albany, Albany, New York 12203, United States.
  • Piorek B; Department of Nanoscale Science & Engineering, University at Albany, Albany, New York 12203, United States.
  • Meinhart C; University of California at Santa Barbara, Santa Barbara, California 93106, United States.
  • Tice J; University of California at Santa Barbara, Santa Barbara, California 93106, United States.
  • Miller BL; QuidelOrtho, Inc., Rochester, New York 14626, United States.
ACS Sens ; 9(4): 1799-1808, 2024 04 26.
Article en En | MEDLINE | ID: mdl-38549498
ABSTRACT
Photonic technologies promise to deliver quantitative, multiplex, and inexpensive medical diagnostic platforms by leveraging the highly scalable processes developed for the fabrication of semiconductor microchips. However, in practice, the affordability of these platforms is limited by complex and expensive sample handling and optical alignment. We previously reported the development of a disposable photonic assay that incorporates inexpensive plastic micropillar microfluidic cards for sample delivery. That system as developed was limited to singleplex assays due to its optical configuration. To enable multiplexing, we report a new approach addressing multiplex light I/O, in which the outputs of individual grating couplers on a photonic chip are mapped to fibers in a fiber bundle. As demonstrated in the context of detecting antibody responses to influenza and SARS-CoV-2 antigens in human serum and saliva, this enables multiplexing in an inexpensive, disposable, and compact format.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Técnicas Biosensibles / SARS-CoV-2 / COVID-19 Límite: Humans Idioma: En Revista: ACS Sens Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Técnicas Biosensibles / SARS-CoV-2 / COVID-19 Límite: Humans Idioma: En Revista: ACS Sens Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos