Protocol for isolating small cytosolic dsDNA from cultured murine cells.
STAR Protoc
; 5(2): 102998, 2024 Jun 21.
Article
en En
| MEDLINE
| ID: mdl-38573862
ABSTRACT
We recently identified a class of small cytosolic double-stranded DNA (scDNA) approximately 20-40 bp in size in human and mouse cells. Here, we present a protocol for scDNA isolation from cultured murine cells. We describe steps for cytosolic compartment separation, DNA isolation in the cytosolic fraction using phenol-chloroform extraction, and ethanol precipitation. We then detail procedures for denaturing purified cytosolic DNA through urea polyacrylamide gel electrophoresis and obtaining scDNA in the cytosolic DNA fraction via gel purification. For complete details on the use and execution of this protocol, please refer to Liu et al.1.
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Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
ADN
/
Citosol
Límite:
Animals
Idioma:
En
Revista:
STAR Protoc
Año:
2024
Tipo del documento:
Article