Proactive vaccination using multiviral Quartet Nanocages to elicit broad anti-coronavirus responses.
Nat Nanotechnol
; 19(8): 1216-1223, 2024 Aug.
Article
en En
| MEDLINE
| ID: mdl-38710880
ABSTRACT
Defending against future pandemics requires vaccine platforms that protect across a range of related pathogens. Nanoscale patterning can be used to address this issue. Here, we produce quartets of linked receptor-binding domains (RBDs) from a panel of SARS-like betacoronaviruses, coupled to a computationally designed nanocage through SpyTag/SpyCatcher links. These Quartet Nanocages, possessing a branched morphology, induce a high level of neutralizing antibodies against several different coronaviruses, including against viruses not represented in the vaccine. Equivalent antibody responses are raised to RBDs close to the nanocage or at the tips of the nanoparticle's branches. In animals primed with SARS-CoV-2 Spike, boost immunizations with Quartet Nanocages increase the strength and breadth of an otherwise narrow immune response. A Quartet Nanocage including the Omicron XBB.1.5 'Kraken' RBD induced antibodies with binding to a broad range of sarbecoviruses, as well as neutralizing activity against this variant of concern. Quartet nanocages are a nanomedicine approach with potential to confer heterotypic protection against emergent zoonotic pathogens and facilitate proactive pandemic protection.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Anticuerpos Neutralizantes
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Glicoproteína de la Espiga del Coronavirus
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Vacunas contra la COVID-19
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SARS-CoV-2
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COVID-19
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Anticuerpos Antivirales
Límite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Nat Nanotechnol
Año:
2024
Tipo del documento:
Article
País de afiliación:
Reino Unido