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Development and characterization of a novel specific monoclonal antibody against mink enteritis virus and its antigen epitope analysis.
Sun, Yuxin; Li, Hongmei; Wang, Haoran; Wang, Ruonan; Kong, Yijia; Chu, Lina; Zhang, Xiying; Li, Hongxin; Lv, Yunwei; Qiu, Jianhua; Guo, Huijun.
Afiliación
  • Sun Y; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.
  • Li H; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.
  • Wang H; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.
  • Wang R; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.
  • Kong Y; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.
  • Chu L; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.
  • Zhang X; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.
  • Li H; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.
  • Lv Y; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.
  • Qiu J; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China. Electronic address: qiujianhua@sdau.edu.cn.
  • Guo H; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China. Electronic address: hjguo@sdau.edu.cn.
Microb Pathog ; 192: 106709, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38810766
ABSTRACT
This study prepared a novel monoclonal antibody (MAb) against mink enteritis parvovirus (MEV) and identified its antigen epitope. The antibody subclass is identified as IgG1, the titers of the MAb is up to 11 × 106 and keeps stably after low-temperature storage for 9 months or 11 passages of the MAb cells. The MAb can specifically recognize MEV in the cells in IFA, but not Aleutian disease virus (ADV) or canine distemper virus (CDV). Its antigen epitope was identified as a polypeptide containing 5 key amino acids (378YAFGR382) and the homology in 20 MEV strains, 4 canine parvovirus strains, and 4 feline panleukopenia virus strains was 100%. This study supplies a biological material for developing new methods to detect MEV.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Virus del Moquillo Canino / Virus de la Enteritis del Visón / Anticuerpos Monoclonales / Anticuerpos Antivirales / Epítopos Límite: Animals Idioma: En Revista: Microb Pathog Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Virus del Moquillo Canino / Virus de la Enteritis del Visón / Anticuerpos Monoclonales / Anticuerpos Antivirales / Epítopos Límite: Animals Idioma: En Revista: Microb Pathog Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China