From Intercalation to External Binding: Ru(II) Complexes with a Spiro Ligand for TAR RNA Selective Binding and HIV-1 Reverse Transcriptase Inhibition.
Inorg Chem
; 63(26): 12342-12349, 2024 Jul 01.
Article
en En
| MEDLINE
| ID: mdl-38904258
ABSTRACT
As a typical RNA virus, the genetic information on HIV-1 is entirely stored in RNA. The reverse transcription activity of HIV-1 reverse transcriptase (RT) plays a crucial role in the replication and transmission of the virus. Non-nucleoside RT inhibitors (NNRTIs) block the function of RT by binding to the RNA binding site on RT, with very few targeting viral RNA. In this study, by transforming planar conjugated ligands into a spiro structure, we convert classical Ru(II) DNA intercalators into a nonintercalator. This enables selective binding to HIV-1 transactivation response (TAR) RNA on the outer side of nucleic acids through dual interactions involving hydrogen bonds and electrostatic attraction, effectively inhibiting HIV-1 RT and serving as a selective fluorescence probe for TAR RNA.
Texto completo:
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Bases de datos:
MEDLINE
Asunto principal:
Rutenio
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VIH-1
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Inhibidores de la Transcriptasa Inversa
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Transcriptasa Inversa del VIH
Límite:
Humans
Idioma:
En
Revista:
Inorg Chem
/
Inorg. chem
/
Inorganic chemistry
Año:
2024
Tipo del documento:
Article