A synthetic TLR4 agonist significantly increases humoral immune responses and the protective ability of an MDCK-cell-derived inactivated H7N9 vaccine in mice.
Arch Virol
; 169(8): 163, 2024 Jul 11.
Article
en En
| MEDLINE
| ID: mdl-38990396
ABSTRACT
Antigenically divergent H7N9 viruses pose a potential threat to public health, with the poor immunogenicity of candidate H7N9 vaccines demonstrated in clinical trials underscoring the urgent need for more-effective H7N9 vaccines. In the present study, mice were immunized with various doses of a suspended-MDCK-cell-derived inactivated H7N9 vaccine, which was based on a low-pathogenic H7N9 virus, to assess cross-reactive immunity and cross-protection against antigenically divergent H7N9 viruses. We found that the CRX-527 adjuvant, a synthetic TLR4 agonist, significantly enhanced the humoral immune responses of the suspended-MDCK-cell-derived H7N9 vaccine, with significant antigen-sparing and immune-enhancing effects, including robust virus-specific IgG, hemagglutination-inhibiting (HI), neuraminidase-inhibiting (NI), and virus-neutralizing (VN) antibody responses, which are crucial for protection against influenza virus infection. Moreover, the CRX-527-adjuvanted H7N9 vaccine also elicited cross-protective immunity and cross-protection against a highly pathogenic H7N9 virus with a single vaccination. Notably, NI and VN antibodies might play an important role in cross-protection against lethal influenza virus infections. This study showed that a synthetic TLR4 agonist adjuvant has a potent immunopotentiating effect, which might be considered worth further development as a means of increasing vaccine effectiveness.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Vacunas contra la Influenza
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Vacunas de Productos Inactivados
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Infecciones por Orthomyxoviridae
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Receptor Toll-Like 4
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Inmunidad Humoral
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Subtipo H7N9 del Virus de la Influenza A
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Ratones Endogámicos BALB C
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Anticuerpos Antivirales
Límite:
Animals
Idioma:
En
Revista:
Arch Virol
Año:
2024
Tipo del documento:
Article
País de afiliación:
China