Protocol for the isolation and proteomic analysis of pathological tau-seeds.
STAR Protoc
; 5(3): 103185, 2024 Jul 29.
Article
en En
| MEDLINE
| ID: mdl-39078738
ABSTRACT
The aggregation and spreading of "tau-seeds" are key for the development and progression of tauopathies, including Alzheimer's disease. Here we describe the steps to isolate and analyze biochemically active tau-seeds from human, mouse, and cell origin. We detail the procedure to isolate soluble tau-seeds by size exclusion chromatography and seeding assay. The isolated tau-seed can be further analyzed to determine the interactome by mass spectrometry. This workflow identifies protein-protein interactors of tau-seeds, providing a useful tool for finding new therapeutic targets. For complete details on the use and execution of this protocol, please refer to Martinez et al.1.
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Bases de datos:
MEDLINE
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En
Revista:
STAR Protoc
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estados Unidos