Insulin binding to human ovaries.
J Clin Endocrinol Metab
; 60(3): 460-3, 1985 Mar.
Article
en En
| MEDLINE
| ID: mdl-3972961
Human ovarian tissue samples were obtained at the time of laparotomy, and plasma membrane fractions were prepared and used in receptor assays. Incubations of the membrane fraction were performed with [125I]insulin (porcine), and Scatchard analysis of binding showed biphasic curves. The high affinity sites had an average concentration of 57.4 +/- 7.9 (+/- SEM) fmol/mg protein and a dissociation constant of 3.5 +/- 0.9 nM (n = 9). Neither affinity nor number of binding sites changed significantly during the menstrual cycle. We conclude that there is high affinity binding of [125I]insulin to human ovarian plasma membranes.
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Bases de datos:
MEDLINE
Asunto principal:
Ovario
/
Receptor de Insulina
Límite:
Female
/
Humans
Idioma:
En
Revista:
J Clin Endocrinol Metab
Año:
1985
Tipo del documento:
Article