Specific immunoreactivity of hybridoma-secreted monoclonal anti-melanoma antibodies to cultured cells and freshly derived human cells.
Cancer Res
; 40(10): 3602-9, 1980 Oct.
Article
en En
| MEDLINE
| ID: mdl-6159966
ABSTRACT
The specificities of monoclonal antibodies against melanoma cells were analyzed using radioimmunoassay, mixed-hemadsorption assay, and quantitative absorption on a variety of malignant and nonmalignant cells. Three of the six hybridoma-secreted antibodies bound to the majority of melanoma cell lines, melanoma tumors, and astrocytoma cell lines as well as to all normal and Epstein-Barr virus-transformed lymphocytes tested. The binding pattern coincides with the presence or absence of the DR antigen on human cells. Conversely, two other antibodies, 19-19 and Nu4B, detected two different antigens common to melanoma and astrocytoma cells only. Cloning of melanoma cells resulted in establishment of DR-positive and DR-negative clones, with the binding of Nu4B antibody retained in all.
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Bases de datos:
MEDLINE
Asunto principal:
Células Cultivadas
/
Melanoma
/
Anticuerpos Antineoplásicos
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Cancer Res
Año:
1980
Tipo del documento:
Article