Extraction of circulating gastrointestinal cancer antigen using solid-phase immunoadsorption system of monoclonal antibody-coupled membrane.
J Immunol Methods
; 66(1): 51-8, 1984 Jan 20.
Article
en En
| MEDLINE
| ID: mdl-6363552
An immunoadsorption system of monoclonal antibody immobilized on a polyolefin alloy fiber is described for extraction of serum gastrointestinal cancer antigen (GICA). Continuous circulation or single passage of plasma from gastrointestinal cancer patients through this antibody-fiber matrix resulted in 90% depletion of circulating GICA in 2 h using 0.6 mg immobilized antibody, and 90% depletion in 5 min using 8 mg antibody. Continual circulation resulted in total GICA removal in both cases. Desorption of antibody or of antibody-containing complexes was minimal. This methodology provides a selective and convenient means of removing any targeted substance by monoclonal antibody from the serum, and thus overcomes many of the shortcomings associated with conventional plasmapheresis.
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Bases de datos:
MEDLINE
Asunto principal:
Técnicas de Inmunoadsorción
/
Neoplasias Gastrointestinales
/
Anticuerpos Monoclonales
/
Antígenos de Neoplasias
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Immunol Methods
Año:
1984
Tipo del documento:
Article