2,4-Diamino-6-hydroxypyrimidine, an inhibitor of tetrahydrobiopterin synthesis, downregulates the expression of iNOS protein and mRNA in primary murine macrophages.
FEBS Lett
; 363(1-2): 69-74, 1995 Apr 17.
Article
en En
| MEDLINE
| ID: mdl-7537228
ABSTRACT
2,4-diamino-6-hydroxy-pyrimidine (DAHP), an inhibitor of GTP cyclohydrolase I, blocks the synthesis of tetrahydrobiopterin (BH4), which is a known cofactor of inducible nitric oxide synthase (iNOS). Previously, DAHP was shown to suppress the production of nitric oxide by cytokine-activated fibroblasts, smooth muscle cells or endothelial cells which could be attributed to its function as a cofactor antagonist. Here, we demonstrate that in interferon-gamma-activated murine peritoneal macrophages DAHP suppresses the expression of iNOS mRNA and protein in a BH4-independent manner and, thus, acts by a novel mechanism.
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Bases de datos:
MEDLINE
Asunto principal:
Biopterinas
/
ARN Mensajero
/
Regulación de la Expresión Génica
/
Aminoácido Oxidorreductasas
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Hipoxantinas
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Macrófagos
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
1995
Tipo del documento:
Article
País de afiliación:
Alemania