Immunocytochemical and ultrastructural characterization of endocrine cells and nerves in the intestine of Rana temporaria.
Tissue Cell
; 25(4): 505-16, 1993 Aug.
Article
en En
| MEDLINE
| ID: mdl-8105559
ABSTRACT
Endocrine cells have been identified in the intestine of the frog Rana temporaria after application of the Grimelius and Masson-Fontana techniques. These endocrine cells were examined using immunocytochemical techniques on paraffin and semithin sections for light microscopy. After testing 19 antisera, 12 immunoreactivities were identified. Numerous serotonin-, somatostatin- and GLP-1-immunoreactive cells; a moderate number of PYY-, glucagon-, VIP-, gastrin/CCK-immunoreactive cells and few human PP-, bombesin-, substance P- and neurotensin-immunoreactive cells were found. VIP- and met-enkephalin were identified in nerve fibers of the muscular layer. Using semithin-thin sections five types of endocrine cells (serotonin-, somatostatin-, gastrin/CCK-, glucagon- and bombesin-immunoreactive cells) have been characterized according to their immunocytochemical reaction and the ultrastructure of the secretory granules.
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Bases de datos:
MEDLINE
Asunto principal:
Células APUD
/
Intestinos
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Tissue Cell
Año:
1993
Tipo del documento:
Article
País de afiliación:
España