The V beta complementarity determining region 1 of a major histocompatibility complex (MHC) class I-restricted T cell receptor is involved in the recognition of peptide/MHC I and superantigen/MHC II complex.
J Exp Med
; 179(4): 1087-97, 1994 Apr 01.
Article
en En
| MEDLINE
| ID: mdl-8145032
ABSTRACT
We investigated the role of the complementarity determining region 1 (CDR1) of T cell receptor (TCR) beta chain both in antigen/major histocompatibility complex I (MHC I) and in superantigen (SAg)/MHC II complex recognition. Residues 26 to 31 of the V beta 10 domain of a TCR derived from an H-2Kd-restricted cytotoxic clone were individually changed to alanine, using site-directed mutagenesis, and the mutated TCR beta chains were transfected along with the wild-type TCR alpha chain into a TCR alpha-beta-T hydridoma. These mutations affected antigen/H-2Kd complex recognition, although to a different extent, as estimated by interleukin 2 production. Certain mutations also affected differently the recognition of two Staphylococcal toxins, exfoliative toxin and Staphylococcal enterotoxin C2, presented by HLA-DR1. Whereas mutation of residues D30 or T31 affect the recognition of both toxins, residues T26, L27, and H29 are critical for the recognition of only one of the SAgs. These observations demonstrate the participation of the CDR1 region in the recognition of peptide/MHC class I as well as SAg/MHC II complexes.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Antígenos H-2
/
Antígenos de Histocompatibilidad Clase II
/
Receptores de Antígenos de Linfocitos T alfa-beta
/
Superantígenos
Límite:
Animals
Idioma:
En
Revista:
J Exp Med
Año:
1994
Tipo del documento:
Article
País de afiliación:
Francia