Photosensitized cross-linking and cleavage of pBR322 and M13 DNA: comparison of 4,4',6-trimethylangelicin and 3-carbethoxypsoralen.
J Photochem Photobiol B
; 22(1): 51-7, 1994 Jan.
Article
en En
| MEDLINE
| ID: mdl-8151456
ABSTRACT
The furocourmarins 3-carbethoxypsoralen (3-CP) and 4,4',6-trimethylangelicin (TMA) were generally believed to be incapable of cross-linking DNA upon irradiation with ultraviolet light. Denaturation of photosensitized pBR322 DNA, either supercoiled or previously linearized with a restriction enzyme, proved that 3-CP was indeed monofunctional, but that TMA produced cross-links. Identical conclusions were reached with double stranded M13 DNA which had been linearized with EcoR 1. Both sensitizers also induced partial DNA cleavage. In contrast to 3-CP, photosensitization with TMA made the DNA resistant to enzymatic cleavage.
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Bases de datos:
MEDLINE
Asunto principal:
Plásmidos
/
Furocumarinas
/
Fármacos Sensibilizantes a Radiaciones
/
Daño del ADN
/
ADN Viral
Idioma:
En
Revista:
J Photochem Photobiol B
Asunto de la revista:
BIOLOGIA
Año:
1994
Tipo del documento:
Article