Suppression of human IgE antibody forming cell responses by IL-6.
J Leukoc Biol
; 54(2): 168-70, 1993 Aug.
Article
en En
| MEDLINE
| ID: mdl-8360595
ABSTRACT
To study the effects of cytokines on human IgE antibody forming cells (AFCs), log phase U266 myeloma cells (3 x 10(3)/ml), which secrete immunoglobulin E (IgE), were cultured for 0-24 h with and without cytokine or with or without antibodies against various cytokines. The numbers of IgE AFCs were determined in ELISPOT assay. We found that interleukin-6 (IL-6) suppressed (to 95%) whereas anti-IL-6 increased (to 148%) the numbers of IgE AFCs and that both worked in a dose-dependent fashion. IL-4 and interferon-gamma (IFN-gamma) also suppressed IgE AFC responses in a dose-dependent fashion. However, antibodies to these cytokines had no effect. In contrast, IFN-alpha increased (to fourfold) the numbers of IgE AFCs in a dose-dependent fashion. The data are the first to show a suppressive effect of IL-6 on human IgE responses and may also suggest a role for IL-6 in the treatment of atopic disease.
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Bases de datos:
MEDLINE
Asunto principal:
Inmunoglobulina E
/
Interleucina-6
/
Tolerancia Inmunológica
/
Células Productoras de Anticuerpos
Límite:
Humans
Idioma:
En
Revista:
J Leukoc Biol
Año:
1993
Tipo del documento:
Article