Cloning and functional analysis of the pmmA gene encoding phosphomannomutase from the photosynthetic prokaryote Prochlorothrix hollandica.
Biochim Biophys Acta
; 1290(3): 210-4, 1996 Aug 13.
Article
en En
| MEDLINE
| ID: mdl-8765122
ABSTRACT
The pmmA gene encoding a bifunctional phosphomannomutase/phosphoglucomutase (PMM/PGM) from the photosynthetic prokaryote, Prochlorothrix hollandica has been cloned and sequenced. The gene encodes a 51827 Da polypeptide 48% identical to the PMM of Azospirillum brasilense, 37% identical to the PGMs of pathogenic Neisseria sp. and 37% identical to the bifunctional AlgC PGM/PMM of Pseudomonas aeruginosa. The pmmA gene encodes an enzyme having both PGM and PMM activities as judged by both enzyme assays and complementation analysis in which the cloned gene partially corrected the pgm-1 mutation of Escherichia coli.
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Bases de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Cianobacterias
/
Fosfotransferasas (Fosfomutasas)
/
Genes Bacterianos
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1996
Tipo del documento:
Article
País de afiliación:
Estados Unidos