Identification and characterization of homologues of the Exocyst component Sec10p.
FEBS Lett
; 404(2-3): 135-9, 1997 Mar 10.
Article
en En
| MEDLINE
| ID: mdl-9119050
ABSTRACT
The SEC10 gene product is a member of the Exocyst complex essential for exocytosis in the budding yeast Saccharomyces cerevisiae. We report here the cloning and characterization of human Sec10p (hSec10p; GenBank accession number U85946). hSec10p is a 77-kDa protein with 23% amino acid identity to yeast Sec10p and 37% identity to a C. elegans protein found in the database. Northern and Western blot analyses indicate that hSec10 has a broad tissue distribution. Immunofluorescence staining of COS cells cotransfected with hSec10p and a mammalian Sec8p demonstrates that these two proteins have an identical distribution in the cell including a localization in the peripheral cytoplasm. These data suggest that hSec10p is a component of the mammalian counterpart of the yeast Exocyst complex essential for post-Golgi traffic.
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Bases de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Proteínas Fúngicas
/
Proteínas de Saccharomyces cerevisiae
/
Exocitosis
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
FEBS Lett
Año:
1997
Tipo del documento:
Article
País de afiliación:
Estados Unidos