Theoretical concepts of on-line liquid chromatographic-biochemical detection systems. I. Detection systems based on labelled ligands.
J Chromatogr A
; 787(1-2): 27-35, 1997 Nov 07.
Article
en En
| MEDLINE
| ID: mdl-9408993
ABSTRACT
A theoretical foundation for on-line coupling of liquid chromatography (LC) with fluororeceptor assays based on fluorescent ligands is presented. Using a recently developed LC-receptor affinity detection (RAD) system as a model, equations are derived which describe the detector response and the signal-to-noise ratio as a function of important biochemical and instrumental parameters. The effect of ligand and label affinity, and receptor concentrations on the detector performance were investigated. It was found that the response of the RAD system is correlated with the affinity of the injected compounds and that the relative affinity order of binding ligands is maintained in the RAD system.
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Bases de datos:
MEDLINE
Asunto principal:
Receptores de Estrógenos
/
Cromatografía Liquida
/
Modelos Químicos
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Chromatogr A
Año:
1997
Tipo del documento:
Article
País de afiliación:
Países Bajos