Correlation between p53 gene mutation, p53 protein labeling and PCNA expression in oral squamous cell carcinomas.
Anticancer Res
; 18(1A): 237-40, 1998.
Article
en En
| MEDLINE
| ID: mdl-9568083
ABSTRACT
BACKGROUND:
The prevalence of oral squamous cell carcinoma (OSCC) among the Black community in South Africa is unacceptably high. The association between p53 protein, and PCNA overexpression and the presence of p53 gene mutations was evaluated. MATERIALS ANDMETHODS:
One hundred and ten formalin-fixed, paraffin-embedded blocks of OSCC were selected for immunohistochemical studies for p53 protein and PCNA expression using the DO-7 and PC10 monoclonal antibodies, respectively. DNA was extracted from fifty-five blocks and exons 5 to 9 of the p53 gene were amplified with nested primers, thereafter sequencing was performed to confirm the presence of mutations detected by single stranded conformational polymorphism.RESULTS:
Fifty-six cases (51%) showed p53 expression, while fourteen mutations (25%) were detected. A significant difference was found between the PCNA index in p53 positive and p53 negative tumors while the mean PCNA index for the tumors with p53 mutations was not significantly different from the tumors without mutations.CONCLUSIONS:
No association between p53 protein overexpression and p53 gene mutations could be demonstrated.
Buscar en Google
Bases de datos:
MEDLINE
Asunto principal:
Neoplasias de la Boca
/
Carcinoma de Células Escamosas
/
Genes p53
/
Proteína p53 Supresora de Tumor
/
Antígeno Nuclear de Célula en Proliferación
Tipo de estudio:
Risk_factors_studies
Límite:
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Anticancer Res
Año:
1998
Tipo del documento:
Article
País de afiliación:
Sudáfrica